This test aims to compare the performance differences of PVDF membranes of different brands through protein adsorption and WB experimental tests.
Protein static adsorption test
Membrane | Absorbance | Adsorption |
Ublot™ 0.22μm PVDF Membrane | 0.2701 | 230μg/cm2 |
Ublot™ 0.22μm PVDF Membrane | 0.2802 | 210μg/cm2 |
Ublot™ 0.22μm PVDF Membrane | 0.2753 | 172μg/cm2 |
K 0.22μm PVDF Membrane | 0.2803 | 117μg/cm2 |
K 0.22μm PVDF Membrane | 0.2789 | 132μg/cm2 |
K0.22μm PVDF Membrane | 0.2768 | 156μg/cm2 |
PVDF Membrane WB Experiment Test
Figure 1
Figure 2
Conclusion
The static protein adsorption of the PVDF membranes tested in this study is as follows: Ublot™ > K.
The Western blot (WB) experimental results of the PVDF membranes tested in this study show that:
(1) The Ublot™ 0.22 μm PVDF membrane demonstrates excellent protein transfer efficiency for proteins of varying molecular weights.
(2) The Ublot™ 0.22 μm PVDF membrane provides well-defined and clear transfer band shapes, high band alignment, and low background staining.
(3) The Ublot™ 0.22 μm PVDF membrane performs effectively when transferring multiple samples simultaneously, with low background, clear bands, and high sensitivity.
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