Name: U-Blot® WTAP Rabbit mAb
SKU: WR5263
Price: 268 USD
Availability: InStock

U-Blot® WTAP Rabbit mAb

Ucallm_WR5263-50

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR5263-100

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Product details

Background:The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012],

Specifications

Size	50μL/100μL
Target	WTAP
Reactivity	Human
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	44kD
MW(Observed)	55kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1505R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	WTAP
Protein Name	Pre-mRNA-splicing regulator WTAP
Other Name	WTAP; / KIAA0105; / Pre-mRNA-splicing regulator WTAP; / Female-lethal; / 2; / D homolog; / hFL; / 2; / D; / WT1-associated protein; / Wilms tumor 1-associating protein
Species	Human
Gene ID	9589
Uniprot	Q15007,
Species.1	Mouse
Gene ID.1	60532
Uniprot.1	Q9ER69
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus speckle . Nucleus, nucleoplasm . Cytoplasm . Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus. .
Tissue Expression	Ubiquitously expressed.
Signaling_pathway
Research Areas
Function	Function:Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.,induction:In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the fl(2)d family.,subunit:Interacts with WT1.,tissue specificity:Ubiquitously expressed.,