Price range: $288.00 through $368.00
Background:This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
| Target | RIPK1 Phospho Ser166 |
| Reactivity | Human |
| Applications | WB,IF,ELISA |
| MW(Calculated) | 76kD |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Modification | Phospho |
| Recommended Dilution Ratio | WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Protein A |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Clonality | Monoclonal |
| Clone Number | PT0757R |
| Specificity | RIPK1 (Phospho Ser166) Antibody detects endogenous levels of RIPK1 protein only when phosphorylated at S166.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):MWsKL |
| Gene Name | RIPK1 RIP RIP1 |
| Protein Name | Receptor-interacting serine/threonine-protein kinase 1 (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP) |
| Other Name | Receptor-interacting serine/threonine-protein kinase 1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP; |
| Organism-1 | Human |
| Gene ID-1 | 8737 |
| SwissProt-1 | Q13546 |
| Organism-2 | Mouse |
| Gene ID-2 | 19766 |
| SwissProt-2 | Q60855 |
| Organism-3 | |
| Gene ID-3 | |
| SwissProt-3 | |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds to the death domain of TNFRSF6 and TRADD. Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RIPK3, UBCE7IP1 isoform 3 (ZIN), EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interacts with MAVS/IPS1., |
| Cellular Localization | Cytoplasm . Cell membrane . |
| Tissue Expression | Leukemic T-cell,T-cell,Umbilical vein endothelial cell, |
| Unnamed: 31 | |
| SwissProt-3.1 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Cellular Localization.1 | Nuclear |
