Need Help? +(1)-866-986-9598
0 My Cart$0.00
Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® TGF β1 Rabbit mAb
SKU: WR4232 Categories: ,

U-Blot® TGF β1 Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4232 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
Western blot analysis of lysates from HepG2 cells, using TGF β1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
WR4232 Western blot analysis of lysates from HepG2 cells, using TGF β1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Rat jejunum
WR4232 Rat jejunum

Price range: $268.00 through $328.00

SKU: WR4232-50
Enter the quantity you would like, or drag the panel to find the quantity and price that best for you
Bulk QuoteAuthorize OEM & ODM

Product details

Background:transforming growth factor beta 1(TGFB1) Homo sapiens This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene i

Specifications

Size50μL/100μL
TargetTGFB1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)44kD
MW(Observed)44kD,13kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:2000-1:5000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM0415R
ImmunogenSynthesized peptide derived from human TGF β1 AA range: 279-390
SpecificityEndogenous
Gene NameTGFB1 TGFB
Protein NameTransforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
Other NameTransforming growth factor beta-1; / TGF-beta-1; / [Cleaved into: Latency-associated peptide; / LAP; / ]
SpeciesHuman
Gene ID7040
UniprotP01137,
Species.1Mouse
Gene ID.121803
Uniprot.1P04202,
Species.2Rat
Gene ID.259086
Uniprot.2P17246
Cellular LocalizationSecreted
Tissue ExpressionHighly expressed in bone (PubMed:11746498, PubMed:17827158). Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA) (PubMed:11746498, PubMed:17827158). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage (PubMed:17827158).
Signaling_pathwayCellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> Intestinal immune network for IgA production;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Inflammatory bowel disease;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction
Research Areas>>MAPK signaling pathway; / >>Cytokine-cytokine receptor interaction; / >>FoxO signaling pathway; / >>Cell cycle; / >>Cellular senescence; / >>TGF-beta signaling pathway; / >>Osteoclast differentiation; / >>Hippo signaling pathway; / >>Th17 cell differentiation; / >>Intestinal immune network for IgA production; / >>Relaxin signaling pathway; / >>Non-alcoholic fatty liver disease; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Leishmaniasis; / >>Chagas disease; / >>Malaria; / >>Toxoplasmosis; / >>Amoebiasis; / >>Tuberculosis; / >>Hepatitis B; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Chronic myeloid leukemia; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Inflammatory bowel disease; / >>Rheumatoid arthritis; / >>Hypertrophic cardiomyopathy; / >>Dilated cardiomyopathy; / >>Diabetic cardiomyopathy
FunctionDisease:Defects in TGFB1 are the cause of Camurati-Engelmann disease (CED) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CED is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.,Function:Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.,induction:Activated in vitro at pH below 3.5 and over 12.5.,online information:TGF beta-1 entry,polymorphism:In post-menopausal Japanese women, the frequency of Leu-10 is higher in subjects with osteoporosis than in controls.,PTM:Glycosylated.,PTM:The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.,similarity:Belongs to the TGF-beta family.,subunit:The inactive form consists of a TGFB1 homodimer non-covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT.,tissue specificity:Highly expressed in bone.,
Please login to post questions

Related products

Immunohistochemical analysis of paraffin-embedded Rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Sku: WR4010
Categories: ,

U-Blot® Glutamine Synthetase Rabbit mAb

0 Reviews
Price range: $268.00 through $328.00
Select options
Sku: WR4018
Categories: ,

U-Blot® CDK6 Rabbit mAb

0 Reviews
Price range: $268.00 through $328.00
Select options
Western blot analysis of lysates from NIH-3T3 cells, using GCLM Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Sku: WR4015
Categories: ,

U-Blot® MMP7 Rabbit mAb

0 Reviews
Price range: $268.00 through $328.00
Select options
Western blot analysis of lysates from K562 cells, using α smooth muscle actin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Sku: WR4021
Categories: ,

U-Blot® α smooth muscle actin Rabbit mAb

0 Reviews
Price range: $268.00 through $328.00
Select options
Western blot analysis of lysates from Hela cells, using Clathrin Heavy Chain 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Sku: WR4016
Categories: ,

U-Blot® Tuberin Rabbit mAb

0 Reviews
Price range: $268.00 through $328.00
Select options
Sku: WM1140
Categories: ,

Acetyl Lysine Monoclonal Antibody [10B10]

0 Reviews
Price range: $288.00 through $368.00
Select options

Customer Reviews

  • Shop Categories
Menu
Need Help? +(1)-866-986-9598
Back to Top
Product has been added to your cart