WR4648 Western Blot analysis of A549 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PHYHD1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
$268.00–$328.00Price range: $268.00 through $328.00
Background:Enables 2-oxoglutarate-dependent dioxygenase activity. [provided by Alliance of Genome Resources, Feb 2025]
Specifications
Size
50μL/100μL
Target
PHYHD1
Reactivity
Human
Applications
WB, IF, ICC, FC
MW(Calculated)
MW(Observed)
32kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Unmodified
Recommended Dilution Ratio
WB 1:1000-5000; ICC/IF 1:100-300; FC 1:100-300
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality
Monoclonal
Clone Number
RM0782R
Immunogen
The specific immunogen used to produce this antibody is proprietary information.
Specificity
Endogenous
Gene Name
PHYHD1
Protein Name
Phytanoyl-CoA dioxygenase domain-containing protein 1; Protein PHYHD1
Other Name
PHYHD1; / Phytanoyl-CoA dioxygenase domain-containing protein 1; / Protein PHYHD1
Species
Human
Gene ID
254295
Uniprot
Q5SRE7,
Species.1
Mouse
Gene ID.1
227696
Uniprot.1
Q9DB26,
Species.2
Rat
Gene ID.2
296621
Uniprot.2
Q5BJP9
Cellular Localization
Tissue Expression
Signaling_pathway
Research Areas
Function
2-oxoglutarate(2OG)-dependent dioxygenase that catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner (PubMed:21530488). However, does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives, implying that it is not directly involved in phytanoyl coenzyme-A metabolism (PubMed:21530488). Does not show detectable activity towards fatty acid CoA thioesters (PubMed:21530488). {ECO:0000269|PubMed:21530488}.; [Isoform 2]: Isoform 2 probably lacks enzyme activity. {ECO:0000269|PubMed:21530488}.; [Isoform 3]: Isoform 3 probably lacks enzyme activity. {ECO:0000269|PubMed:21530488}.