Product details

Background:
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016],

Specifications

Size	50μL/100μL
Target	SREBP-1
Reactivity	Human, Mouse, Rat
Applications	WB, IF, ELISA
MW(Calculated)	121kD
MW(Observed)	140kD
Host Species	Mouse
Isotype	IgG3,kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMMR1391
Immunogen	AA range: 1-100
Specificity	This antibody detects endogenous levels of SREBP-1 protein.
Gene Name	SREBF1 BHLHD1 SREBP1
Protein Name	SREBP-1
Other Name	Sterol regulatory element-binding protein 1 (SREBP-1) (Class D basic helix-loop-helix protein 1) (bHLHd1) (Sterol regulatory element-binding transcription factor 1) [Cleaved into: Processed sterol regulatory element-binding protein 1]
Species	Human
Gene ID	6720
Uniprot	P36956,
Species.1	Mouse
Gene ID.1	20787
Uniprot.1	Q9WTN3
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Membrane
Tissue Expression	Expressed in a wide variety of tissues, most abundant in liver and adrenal gland (PubMed:8402897). In fetal tissues lung and liver shows highest expression (PubMed:8402897).; [Isoform SREBP-1A]: Predominates in hepatoma cell lines (PubMed:8402897). Also expressed in kidney, brain, white fat, and muscle (PubMed:8402897).; [Isoform SREBP-1C]: Predominantly expressed in liver and adipose tissues (PubMed:8402897). Also expressed in kidney, brain, white fat, and muscle (PubMed:8402897).
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>AMPK signaling pathway; / >>Insulin signaling pathway; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease
Function	Alternative products:Additional isoforms seem to exist,Function:Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').,online information:Sterol regulatory element-binding protein entry,PTM:At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.,sequence Caution:Intron retention.,similarity:Belongs to the SREBP family.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subcellular location:Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.,subunit:Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.,tissue specificity:Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.,

Please login to post questions

SREBP-1 [PTR1391] mouse mAb

Product details

Specifications

About Us

Support

Order

Menu

SREBP-1 [PTR1391] mouse mAb

Product details

Specifications

Related products

U-Blot® Tuberin Rabbit mAb

U-Blot® BID Rabbit mAb

U-Blot® CD86 Rabbit mAb

U-Blot® GAPDH Rabbit mAb

U-Blot® Hsp90 Rabbit mAb

U-Blot® GSK3 β (Phospho Ser9) Rabbit mAb

Customer Reviews

Menu