Price range: $268.00 through $328.00
Background:
prohibitin(PHB) Homo sapiens This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
| Size | 50μL/100μL |
| Target | Prohibitin |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 30kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 1E2 |
| Immunogen | Purified recombinant fragment of human Prohibitin expressed in E. Coli. |
| Specificity | Prohibitin Monoclonal Antibody detects endogenous levels of Prohibitin protein. |
| Gene Name | PHB |
| Protein Name | Prohibitin |
| Other Name | PHB; / Prohibitin |
| Species | Human |
| Gene ID | 5245 |
| Uniprot | P35232, |
| Species.1 | Mouse |
| Gene ID.1 | 18673 |
| Uniprot.1 | P67778, |
| Species.2 | Rat |
| Gene ID.2 | 25344 |
| Uniprot.2 | P67779 |
| Cellular Localization | Mitochondrion inner membrane . Nucleus . Cytoplasm . Cell membrane . |
| Tissue Expression | Widely expressed in different tissues. |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Levels of expression in fibroblasts decrease heterogeneously during cellular aging.,Disease:Mutated in sporadic breast cancer.,Function:Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.,induction:Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol.,similarity:Belongs to the prohibitin family.,subunit:Interacts with PHB2.,tissue specificity:Widely expressed in different tissues., |
