SKU: WP11112 Categories: ,

Arfaptin 1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11112-100
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Product details

Background:

function:Putative target protein of ADP-ribosylation factor., similarity:Contains 1 AH domain., subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6., tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,

Specifications

TargetArfaptin 1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)40kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human ARFIP1. AA range:271-320
Sequence
SpecificityArfaptin 1 Polyclonal Antibody detects endogenous levels of Arfaptin 1 protein.
Gene NameARFIP1
Protein NameArfaptin-1
Other NameARFIP1; / Arfaptin-1; / ADP-ribosylation factor-interacting protein 1
SpeciesHuman
Gene ID-127236
UniprotP53367,
Species.1
Gene ID-2
Uniprot.1
Species.2Rat
Gene ID-360382
Uniprot.2Q9JHU5
Organism-4
Gene ID-4
SwissProt-4
Backgroundfunction:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,
Cellular LocalizationGolgi apparatus . Golgi apparatus, trans-Golgi network membrane .
Tissue ExpressionUbiquitously expressed (PubMed:9038142). Higher levels in liver, pancreas, placenta, skeletal muscle and heart (PubMed:9038142).
Signaling_pathway
Research Areas
FunctionFunction:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,
RRID
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