Product details

Background:

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011],

Specifications

Target	XIAP
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	57kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human XIAP. AA range:53-102
Sequence
Specificity	XIAP Polyclonal Antibody detects endogenous levels of XIAP protein.
Gene Name	XIAP
Protein Name	E3 ubiquitin-protein ligase XIAP
Other Name	XIAP; / API3; / BIRC4; / IAP3; / E3 ubiquitin-protein ligase XIAP; / Baculoviral IAP repeat-containing protein 4; / IAP-like protein; / ILP; / hILP; / Inhibitor of apoptosis protein 3; / IAP-3; / hIAP-3; / hIAP3; / X-linked inhibitor of apoptosis protein; / X-linked I
Species	Human
Gene ID-1	331
Uniprot	P98170,
Species.1	Mouse
Gene ID-2	11798
Uniprot.1	Q60989,
Species.2	Rat
Gene ID-3	63879
Uniprot.2	Q9R0I6
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011],
Cellular Localization	Cytoplasm. Nucleus. TLE3 promotes its nuclear localization.
Tissue Expression	Expressed in colonic crypts (at protein level) (PubMed:30389919). Ubiquitous, except peripheral blood leukocytes (PubMed:8654366).
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Apoptosis - multiple species;Cellular Processes >> Cell growth and death >> Necroptosis;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis
Research Areas	>>Platinum drug resistance; / >>NF-kappa B signaling pathway; / >>Ubiquitin mediated proteolysis; / >>Apoptosis; / >>Apoptosis - multiple species; / >>Necroptosis; / >>Focal adhesion; / >>NOD-like receptor signaling pathway; / >>Toxoplasmosis; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Small cell lung cancer
Function	Disease:Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.,Domain:The first BIR domain is involved in interaction with MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.,Function:Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.,online information:XIAP mutation db,PTM:Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.,PTM:Ubiquitinated and degraded by the proteasome in apoptotic cells.,similarity:Belongs to the IAP family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 3 BIR repeats.,subunit:Monomer, and homodimer. Interacts with SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with MAP3K7IP1 and AIFM1. Interaction with SMAC hinders binding of MAP3K7IP1 and AIFM1. Interacts with TCF25.,tissue specificity:Ubiquitous, except peripheral blood leukocytes.,
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XIAP Polyclonal Antibody

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XIAP Polyclonal Antibody

Product details

Specifications

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