EEA1 Rabbit Monoclonal Antibody

EEA1 Rabbit Monoclonal Antibody

Background:disease:Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.,domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate., function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.,similarity:Contains 1 C2H2-type zinc finger.,similarity:
Contains 1 FYVE-type zinc finger.,subunit:Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.,

Item Details
EEA1 Rabbit mAb WR4097
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:2500 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 162 kDa
Observed band size 162 kDa

Elevate your endosomal trafficking and autoimmune disease research with the EEA1 Rabbit Monoclonal Antibody (WR4097)—a Google-optimized, rigorously validated tool for specific EEA1 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers perfect band alignment (162 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated detection system.

Ideal for studying phosphatidylinositol 3-phosphate binding, RAB protein interactions, endosomal transport, or autoimmune disease-related EEA1 antibodies (e.g., subacute cutaneous lupus erythematosus), this trusted EEA1 Rabbit Monoclonal Antibody boosts assay reproducibility. Order WR4097 today to advance your EEA1-focused experiments with a SEO-verified, high-performance research tool tailored for cell biology and

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