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Monoclonal Antibody

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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
Sku: WR4467
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U-Blot® Smad1 Rabbit mAb

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Sku: WR4011
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U-Blot® Smad2 (Phospho Ser250) Rabbit mAb

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MCF7 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Sku: WR4508
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U-Blot® SMAD2 (Phospho Ser465/467) Rabbit mAb

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Sku: WR4044
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U-Blot® Smad2 Rabbit mAb

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NIH-3T3 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Sku: WR4519
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U-Blot® Smad2/3 (Phospho Thr8) Rabbit mAb

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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
Sku: WR4635
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U-Blot® Smad3 (Phospho Ser423/Ser425) Rabbit mAb

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Hela-2 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Sku: WR4553
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U-Blot® Smad3 Rabbit mAb

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C6 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Sku: WR4343
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U-Blot® Smad4 Rabbit mAb

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Western Blot analysis using HeLa  lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Sku: WR4531
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U-Blot® SMAD5 (Phospho Ser463/Ser465) Rabbit mAb

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Mouse thymus whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Sku: WR4396
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U-Blot® Smad5 Rabbit mAb

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Sku: WR5010
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U-Blot® SMARCC1 Rabbit mAb

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Sku: WR4970
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U-Blot® SMC1 Rabbit mAb

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