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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/4E-BP1 Polyclonal Antibody
SKU: WP10859 Categories: ,

4E-BP1 Polyclonal Antibody

Ucallm_WP10859-50

Price range: $268.00 through $328.00

SKU: WP10859-100
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Product details

Background:

eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
Target4E-BP1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)13kD
MW(Observed)18kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:4-53
Specificity4E-BP1 Polyclonal Antibody detects endogenous levels of 4E-BP1 protein.
Gene NameEIF4EBP1
Protein NameEukaryotic translation initiation factor 4E-binding protein 1
Other NameEIF4EBP1; / Eukaryotic translation initiation factor 4E-binding protein 1; / 4E-BP1; / eIF4E-binding protein 1; / Phosphorylated heat- and acid-stable protein regulated by insulin 1; / PHAS-I
SpeciesHuman
Gene ID1978
UniprotQ13541,
Species.1Mouse
Gene ID.113685
Uniprot.1Q60876,
Species.2Rat
Gene ID.2116636
Uniprot.2Q62622
Cellular LocalizationNucleus
Tissue ExpressionColon,Epithelium,Lung,Placenta,Platelet,
Signaling_pathwayCellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas>>EGFR tyrosine kinase inhibitor resistance; / >>ErbB signaling pathway; / >>HIF-1 signaling pathway; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Cellular senescence; / >>Insulin signaling pathway; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Herpes simplex virus 1 infection; / >>Chemical carcinogenesis - receptor activation; / >>Acute myeloid leukemia; / >>Choline metabolism in cancer
FunctionFunction:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,
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