Name: U-Blot® 53BP2/ASPP2 Rabbit mAb
SKU: WR4697
Price: 268 USD
Availability: InStock

U-Blot® 53BP2/ASPP2 Rabbit mAb

Western Blot analysis of HEK-293T whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-53BP2/ASPP2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4697 Western Blot analysis of HEK-293T whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-53BP2/ASPP2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4697-50

Enter the quantity you would like, or drag the panel to find the quantity and price that best for you

Bulk Quote Authorize OEM & ODM

Product details

Background:This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	ASPP2
Reactivity	Human
Applications	WB, IHC, IF, ICC
MW(Calculated)
MW(Observed)	124kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000-5000; IHC 1:100-300; ICC/IF 1:100-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0791R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	TP53BP2; ASPP2; BBP
Protein Name	Apoptosis-stimulating of p53 protein 2
Other Name	TP53BP2; / ASPP2; / BBP; / Apoptosis-stimulating of p53 protein 2; / Bcl2-binding protein; / Bbp; / Renal carcinoma antigen NY-REN-51; / Tumor suppressor p53-binding protein 2; / 53BP2; / p53-binding protein 2; / p53BP2
Species	Human
Gene ID	7159
Uniprot	Q13625,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q8CG79
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm, perinuclear region. Nucleus. Note=Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
Tissue Expression	Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines. {ECO:0000269\|PubMed:10498867, ECO:0000269\|PubMed:10631318, ECO:0000269\|PubMed:11684014}.
Signaling_pathway	Environmental Information Processing >> Signal transduction >> Hippo signaling pathway
Research Areas	>>Hippo signaling pathway
Function	Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53 (PubMed:12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of NAE1 to conjugate NEDD8 to CUL1, and thereby decreases NAE1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42. {ECO:0000269\|PubMed:11684014, ECO:0000269\|PubMed:12524540, ECO:0000269\|PubMed:12694406, ECO:0000269\|PubMed:19377511}.