U-Blot® Acetyl Coenzyme A Carboxylase beta Rabbit mAb
WR4379 PC-12 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | ACC-alpha |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 265kD |
| MW(Observed) | 265kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0598R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACACA |
| Protein Name | Acetyl-CoA carboxylase 1; ACC1; Acetyl-Coenzyme A carboxylase alpha; ACC-alpha |
| Other Name | ACACA; / ACAC; / ACC1; / ACCA; / Acetyl-CoA carboxylase 1; / ACC1; / ACC-alpha |
| Species | Human |
| Gene ID | 31 |
| Uniprot | Q13085, |
| Species.1 | Mouse |
| Gene ID.1 | 107476 |
| Uniprot.1 | Q5SWU9, |
| Species.2 | Rat |
| Gene ID.2 | 60581 |
| Uniprot.2 | P11497 |
| Cellular Localization | Cytoplasm, cytosol . |
| Tissue Expression | Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>Fatty acid biosynthesis; / >>Pyruvate metabolism; / >>Propanoate metabolism; / >>Metabolic pathways; / >>Fatty acid metabolism; / >>AMPK signaling pathway; / >>Insulin signaling pathway; / >>Glucagon signaling pathway; / >>Alcoholic liver disease |
| Function | Catalytic activity:ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA.,Catalytic activity:ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.,cofactor:Binds 2 manganese ions per subunit.,cofactor:Biotin.,Disease:Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.,enzyme regulation:By phosphorylation.,Function:Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.,online information:Acetyl-CoA carboxylase entry,pathway:Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.,PTM:Phosphorylation on Ser-1263 is required for interaction with BRCA1.,similarity:Contains 1 ATP-grasp domain.,similarity:Contains 1 biotin carboxylation domain.,similarity:Contains 1 biotinyl-binding domain.,similarity:Contains 1 carboxyltransferase domain.,subunit:Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.,tissue specificity:Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver., |
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