Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010],
| Target | ACOT8 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 36kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT8. AA range:131-180 |
| Sequence | |
| Specificity | ACOT8 Polyclonal Antibody detects endogenous levels of ACOT8 protein. |
| Gene Name | ACOT8 |
| Protein Name | Acyl-coenzyme A thioesterase 8 |
| Other Name | ACOT8; / ACTEIII; / PTE1; / PTE2; / Acyl-coenzyme A thioesterase 8; / Acyl-CoA thioesterase 8; / Choloyl-coenzyme A thioesterase; / HIV-Nef-associated acyl-CoA thioesterase; / PTE-2; / Peroxisomal acyl-coenzyme A thioester hydrolase 1; / PTE-1; / Peroxisomal lon |
| Species | Human |
| Gene ID-1 | 10005 |
| Uniprot | O14734, |
| Species.1 | Mouse |
| Gene ID-2 | |
| Uniprot.1 | P58137 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010], |
| Cellular Localization | Peroxisome matrix . Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded. . |
| Tissue Expression | Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485). |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome |
| Research Areas | >>Primary bile acid biosynthesis; / >>Metabolic pathways; / >>Peroxisome |
| Function | Catalytic activity:Choloyl-CoA + H(2)O = cholate + CoA.,Function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation.,induction:Regulated by peroxisome proliferator (such as Clofibrate), via the peroxisome proliferator-activated receptors (PPARs).,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Interacts with HIV-1 Nef.,tissue specificity:Detected in a T-cell line (at protein level). Ubiquitous., |
| RRID |
