Price range: $268.00 through $328.00
Background:
Smooth muscle actin is a part of actin family of proteins which is specific in smooth muscle. The antibody can label smooth muscle actin but no cross reaction with actin of skeletal muscle and myocardium. It is mainly used to label smooth muscle and myoepithelial cells and tumors of their origin.
| Size | 50μL/100μL |
| Target | Actin_smooth muscle (SMA) |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 42kD |
| Host Species | Mouse |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-SMA |
| Immunogen | Synthesized peptide derived from human Actin, smooth muscle (SMA) AA range: 1-100 |
| Specificity | The antibody can specifically recognize smooth muscle actin and shows no reaction with actin of skelatal muscle or cardiac muscle. |
| Gene Name | ACTA2/ACTG2 |
| Protein Name | Actin, smooth muscle |
| Other Name | |
| Species | Human |
| Gene ID | |
| Uniprot | P62736;P63267 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Pituitary,Uterus, |
| Signaling_pathway | |
| Research Areas | >>Vascular smooth muscle contraction; / >>Apelin signaling pathway; / >>Relaxin signaling pathway |
| Function | Disease:Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.,Function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the actin family.,subunit:Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others., |
