Adducin α/β Polyclonal Antibody
Ucallm_WP10960-50
Price range: $268.00 through $328.00
Product details
Background:
adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Adducin α/β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IP, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IP 2-5 ug/mg lysate; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADD1. AA range:688-737 |
| Specificity | Adducin α/β Polyclonal Antibody detects endogenous levels of Adducin α/β protein. |
| Gene Name | ADD1/ADD2 |
| Protein Name | Alpha-adducin/Beta-adducin |
| Other Name | ADD1; / ADDA; / Alpha-adducin; / Erythrocyte adducin subunit alpha; / ADD2; / ADDB; / Beta-adducin; / Erythrocyte adducin subunit beta |
| Species | Human |
| Gene ID | 118;119 |
| Uniprot | P35611;P35612, |
| Species.1 | Mouse |
| Gene ID.1 | 11518;11519, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 24170;24171 |
| Uniprot.2 | Q63028;Q05764 |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
| Tissue Expression | Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. |
| Signaling_pathway | |
| Research Areas | |
| Function | Alternative products:Additional isoforms seem to exist,Domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,Function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit., |
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