AMPKα1 Polyclonal Antibody
Ucallm_WP11032-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | AMPKα1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 65kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AMPK1. AA range:451-500 |
| Specificity | AMPKα1 Polyclonal Antibody detects endogenous levels of AMPKα1 protein. |
| Gene Name | PRKAA1 |
| Protein Name | 5'-AMP-activated protein kinase catalytic subunit alpha-1 |
| Other Name | PRKAA1; / AMPK1; / 5'-AMP-activated protein kinase catalytic subunit alpha-1; / AMPK subunit alpha-1; / Acetyl-CoA carboxylase kinase; / ACACA kinase; / Hydroxymethylglutaryl-CoA reductase kinase; / HMGCR kinase; / Tau-protein kinase PRKAA1 |
| Species | Human |
| Gene ID | 5562 |
| Uniprot | Q13131, |
| Species.1 | Mouse |
| Gene ID.1 | 105787 |
| Uniprot.1 | Q5EG47, |
| Species.2 | Rat |
| Gene ID.2 | 65248 |
| Uniprot.2 | P54645 |
| Cellular Localization | Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. . |
| Tissue Expression | Brain,Intestine,Liver,Mammary gland,Platelet,Testis |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Organismal Systems >> Environmental adaptation >> Circadian rhythm;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Apelin signaling pathway; / >>Tight junction; / >>Circadian rhythm; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Adipocytokine signaling pathway; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease; / >>Hypertrophic cardiomyopathy; / >>Fluid shear stress and atherosclerosis |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,Function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,sequence Caution:Translation N-terminally shortened.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2., |
Please login to post questions
Related products
