SKU: WR4939 Categories: ,

U-Blot® ATF-3 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR4939-50
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Product details

Background:activating transcription factor 3(ATF3) Homo sapiens This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011],

Specifications

Size50μL/100μL
TargetATF-3
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)21kD
MW(Observed)21kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1162R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameATF3
Protein NameCyclic AMP-dependent transcription factor ATF-3
Other NameATF3; / Cyclic AMP-dependent transcription factor ATF-3; / cAMP-dependent transcription factor ATF-3; / Activating transcription factor 3
SpeciesHuman
Gene ID467
UniprotP18847,
Species.1Mouse
Gene ID.111910
Uniprot.1Q60765
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus .
Tissue ExpressionAmygdala,Brain,Cerebellum,Muscle,
Signaling_pathway
Research Areas
FunctionFunction:This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a homodimer or a heterodimer.,
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