ATF-6β Polyclonal Antibody
Ucallm_WP11187-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008],
Specifications
| Size | 50μL/100μL |
| Target | ATF-6β |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 90kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:20000; IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATF6B. AA range:401-450 |
| Specificity | ATF-6β Polyclonal Antibody detects endogenous levels of ATF-6β protein. |
| Gene Name | ATF6B |
| Protein Name | Cyclic AMP-dependent transcription factor ATF-6 beta |
| Other Name | ATF6B; / CREBL1; / G13; / Cyclic AMP-dependent transcription factor ATF-6 beta; / cAMP-dependent transcription factor ATF-6 beta; / Activating transcription factor 6 beta; / ATF6-beta; / Protein G13; / cAMP response element-binding protein-related protein |
| Species | Human |
| Gene ID | 1388 |
| Uniprot | Q99941, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O35451 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein .; [Processed cyclic AMP-dependent transcription factor ATF-6 beta]: Nucleus . Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. . |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Aging >> Longevity regulating pathway;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>cGMP-PKG signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>PI3K-Akt signaling pathway; / >>Longevity regulating pathway; / >>Adrenergic signaling in cardiomyocytes; / >>TNF signaling pathway; / >>Dopaminergic synapse; / >>Insulin secretion; / >>Estrogen signaling pathway; / >>Thyroid hormone synthesis; / >>Aldosterone synthesis and secretion; / >>Relaxin signaling pathway; / >>Cortisol synthesis and secretion; / >>Parathyroid hormone synthesis, secretion and action; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Prion disease; / >>Cocaine addiction; / >>Amphetamine addiction; / >>Alcoholism; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Viral carcinogenesis; / >>Chemical carcinogenesis - receptor activation |
| Function | Domain:The basic domain functions as a nuclear localization signal.,Domain:The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.,Function:Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN(9)CCAC[GA]-3') when NF-Y is bound to ERSE.,PTM:During unfolded protein response an approximative 60 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is probably performed sequentially by site-1 and site-2 proteases.,PTM:N-glycosylated.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subcellular location:Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.,subunit:Homodimer and heterodimer with ATF6-alpha. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC).,tissue specificity:Ubiquitous., |
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