Name: U-Blot® Caspase-6 Rabbit mAb
SKU: WR4575
Price: 268 USD
Availability: InStock

U-Blot® Caspase-6 Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4575 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

A431 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4575 A431 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4575-50

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Product details

Background:This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015],

Specifications

Size	50μL/100μL
Target	Caspase 6
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	33kD
MW(Observed)	33kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0513R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	CASP6
Protein Name	Caspase6
Other Name	CASP6; / MCH2; / Caspase-6; / CASP-6; / Apoptotic protease Mch-2
Species	Human
Gene ID	84152
Uniprot	P55212,
Species.1	Mouse
Gene ID.1	12368
Uniprot.1	O08738,
Species.2	Rat
Gene ID.2	360616
Uniprot.2	Q6J4I0
Cellular Localization	Cytoplasm . Nucleus .
Tissue Expression	Adipose tissue,Brain,Cerebellum,Colon,Ovary,
Signaling_pathway	Organismal Systems >> Nervous system >> Dopaminergic synapse;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>cAMP signaling pathway; / >>Dopaminergic synapse; / >>Cocaine addiction; / >>Amphetamine addiction; / >>Alcoholism
Function	Catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-\|-.,enzyme regulation:Activation is suppressed by phosphorylation at Ser-257.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.,PTM:Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit.,