Name: U-Blot® NEDD4 Rabbit mAb
SKU: WR5109
Price: 268 USD
Availability: InStock

U-Blot® NEDD4 Rabbit mAb

Ucallm_WR5109-50

Price range: $268.00 through $328.00

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50μl

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SKU: WR5109-50

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Product details

Background:This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016],

Specifications

Size	50μL/100μL
Target	NEDD4
Reactivity	Human, Mouse, Rat
Applications	WB, IF, IP, ELISA
MW(Calculated)	149kD
MW(Observed)	130kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1292R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	NEDD4 KIAA0093 NEDD4-1 PIG53
Protein Name	NEDD4
Other Name
Species	Human
Gene ID	4734
Uniprot	P46934,
Species.1	Mouse
Gene ID.1	17999
Uniprot.1	P46935,
Species.2	Rat
Gene ID.2	25489
Uniprot.2	Q62940
Cellular Localization	Cytoplasm . Cell membrane; Peripheral membrane protein . Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. .
Tissue Expression
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis
Research Areas	>>Ubiquitin mediated proteolysis; / >>Endocytosis; / >>Tight junction; / >>Epstein-Barr virus infection
Function	Domain:The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.,Function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Involved in the budding of many viruses.,miscellaneous:A cysteine residue is required for ubiquitin-thioester formation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2 (By similarity). Interacts with viral proteins that contain a late-budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag.,