CD110 Polyclonal Antibody
Ucallm_WP15497-50
Price range: $268.00 through $328.00
Product details
Background:
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important
Specifications
| Size | 50μL/100μL |
| Target | TPOR |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 69,40kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from Thrombopoietin receptor at AA range: 321-370 |
| Specificity | CD110 Polyclonal Antibody detects endogenous levels of CD110 protein. |
| Gene Name | MPL TPOR |
| Protein Name | Thrombopoietin receptor |
| Other Name | MPL; / TPOR; / Thrombopoietin receptor; / TPO-R; / Myeloproliferative leukemia protein; / Proto-oncogene c-Mpl; / CD110 |
| Species | Human |
| Gene ID | 4352 |
| Uniprot | P40238, |
| Species.1 | Mouse |
| Gene ID.1 | 17480 |
| Uniprot.1 | Q08351 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Golgi apparatus . Cell surface . |
| Tissue Expression | Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction |
| Research Areas | >>Cytokine-cytokine receptor interaction; / >>JAK-STAT signaling pathway |
| Function | Caution:It is uncertain whether Met-1 or Met-8 is the initiator.,Disease:Defects in MPL are a cause of congenital amegakaryocytic thrombocytopenia (CAMT) [MIM:604498]. CAMT is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.,Domain:The box 1 motif is required for JAK interaction and/or activation.,Domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,Function:Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.,similarity:Belongs to the type I cytokine receptor family. Type 1 subfamily.,similarity:Contains 2 fibronectin type-III domains.,subunit:Interacts with ATXN2L.,tissue specificity:Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed., |
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