Name: CD117 [C-kit] [ABT233] mouse mAb
SKU: WM1466
Price: 268 USD
Availability: InStock

Product details

Background:
CD117 is a cell membrane protein encoded by the c-kit proto-oncogene and is a type III transmembrane protein tyrosine kinase growth factor receptor. CD117 is expressed in mast cells, melanocytes and interstitial cells of Cajal. CD117 is positive in 80-100% of gastric stromal sarcoma (GIST). CD117, as a specific marker for immunohistochemical examination of gastric stromal sarcoma (GIST), can be used to clinically guide the personalized target drug (Gleevec) treatment of GIST patients. CD117 can also be used to identify mast cell tumors in lymphoma and as a marker for some testicular germ cell tumors.

Specifications

Size	50μL/100μL
Target	CD117
Reactivity	Human
Applications	IHC, WB, IF, ELISA
MW(Calculated)	120kD
MW(Observed)	150kD
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM233
Immunogen	Synthesized peptide derived from human CD117 AA range: 300-400
Specificity	The antibody can specifically recognize human CD117 protein.
Gene Name	KIT SCFR
Protein Name	C Kit; c-Kit; c-Kit Ligand; CD117; Kit; Kit Ligand; KIT oncogene; KIT proto oncogene receptor tyrosine kinase; KIT_HUMAN; Mast cell growth factor receptor; Mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; Proto oncogene c Kit; Proto oncogene tyrosine protein kinase Kit; Proto-oncogene c-Kit; SCF Receptor; SCFR; soluble KIT variant 1; Steel Factor Receptor; Stem cell factor receptor; tyrosine protein kinase Kit; Tyrosine-protein kinase Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Other Name	C Kit; / c-Kit; / c-Kit Ligand; / CD117; / Kit; / Kit Ligand; / KIT oncogene; / KIT proto oncogene receptor tyrosine kinase; / KIT_HUMAN; / Mast cell growth factor receptor; / Mast/stem cell growth factor receptor Kit; / MGF; / p145 c-kit; / PBT; / Piebald trait protein; / Proto oncogene c Kit; / Proto oncogene tyrosine protein kinase Kit; / Proto-oncogene c-Kit; / SCF Receptor; / SCFR; / soluble KIT variant 1; / Steel Factor Receptor; / Stem cell factor receptor; / tyrosine protein kinase Kit; / Tyrosine-protein kinase Kit; / v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; / v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein; / v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Species	Human
Gene ID	3815
Uniprot	P10721,
Species.1	Mouse
Gene ID.1	16590
Uniprot.1	P05532
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic, Membranous
Tissue Expression	Appendix
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Breast cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>Phospholipase D signaling pathway; / >>PI3K-Akt signaling pathway; / >>Hematopoietic cell lineage; / >>Melanogenesis; / >>Pathways in cancer; / >>Acute myeloid leukemia; / >>Breast cancer; / >>Central carbon metabolism in cancer
Function	Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Disease:Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764].,Disease:Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes.,Disease:Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).,Function:This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).,online information:CD117 entry,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts with APS. Interacts with MPDZ (via the tenth PDZ domain). Interacts with PTPRU.,