U-Blot® CD14 Rabbit mAb
WR4234 Western blot analysis of lysates from RAW264.7 cells, using CD14 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
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Product details
Background:The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010],
Specifications
| Size | 50μL/100μL |
| Target | CD14 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 39kD |
| MW(Observed) | 52kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:400-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0417R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CD14 |
| Protein Name | CD14 |
| Other Name | Monocyte differentiation antigen CD14; / Myeloid cell-specific leucine-rich glycoprotein; / CD antigen CD14; / [Cleaved into: Monocyte differentiation antigen CD14, urinary form; / Monocyte differentiation antigen CD14, membrane-bound form] |
| Species | Human |
| Gene ID | 929 |
| Uniprot | P08571, |
| Species.1 | Mouse |
| Gene ID.1 | 12475 |
| Uniprot.1 | P10810, |
| Species.2 | Rat |
| Gene ID.2 | 60350 |
| Uniprot.2 | Q63691 |
| Cellular Localization | Membrane |
| Tissue Expression | Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>NF-kappa B signaling pathway; / >>Phagosome; / >>Toll-like receptor signaling pathway; / >>Hematopoietic cell lineage; / >>Alcoholic liver disease; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Amoebiasis; / >>Tuberculosis; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia; / >>Lipid and atherosclerosis |
| Function | Function:Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.,online information:CD14 entry,similarity:Contains 11 LRR (leucine-rich) repeats.,subunit:Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.,tissue specificity:Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages., |
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