Name: CD42d/GPV Polyclonal Antibody
SKU: WP15589
Price: 268 USD
Availability: InStock

Product details

Background:

Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome (MIM 231200), a bleeding disorder (review by Lopez et al., 1998 [PubMed 9616133]).[supplied by OMIM, Nov 2010],

Specifications

Target	CD42d
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)
MW(Observed)	62kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from the Internal region of human GP5. AA range:331-380
Sequence
Specificity	CD42d Polyclonal Antibody detects endogenous levels of CD42d protein.
Gene Name	GP5
Protein Name	Platelet glycoprotein V
Other Name	GP5; / Platelet glycoprotein V; / GPV; / Glycoprotein 5; / CD42d
Species	Human
Gene ID-1	2814
Uniprot	P40197,
Species.1	Mouse
Gene ID-2
Uniprot.1	O08742,
Species.2	Rat
Gene ID-3	25259
Uniprot.2	O08770
Organism-4
Gene ID-4
SwissProt-4
Background	Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome (MIM 231200), a bleeding disorder (review by Lopez et al., 1998 [PubMed 9616133]).[supplied by OMIM, Nov 2010],
Cellular Localization	Membrane; Single-pass type I membrane protein.
Tissue Expression	Platelets and megakaryocytes.
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Platelet activation;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction
Research Areas	>>ECM-receptor interaction; / >>Platelet activation; / >>Hematopoietic cell lineage
Function	Function:The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.,PTM:The N-terminus is blocked.,similarity:Contains 14 LRR (leucine-rich) repeats.,tissue specificity:Platelets and megakaryocytes.,
RRID