U-Blot® CD81 Rabbit mAb
WR4496 Western Blot analysis using Neuro-2a lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],
Specifications
| Size | 50μL/100μL |
| Target | CD81 |
| Reactivity | Human, Mouse, Rat, Bovine |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 26kD |
| MW(Observed) | 22kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0717R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CD81 |
| Protein Name | CD81 antigen |
| Other Name | CD81; / TAPA1; / TSPAN28; / CD81 antigen; / 26 kDa cell surface protein TAPA-1; / Target of the antiproliferative antibody 1; / Tetraspanin-28; / Tspan-28; / CD81 |
| Species | Human |
| Gene ID | 975 |
| Uniprot | P60033, |
| Species.1 | Mouse |
| Gene ID.1 | 12520 |
| Uniprot.1 | P35762, |
| Species.2 | Rat |
| Gene ID.2 | 25621 |
| Uniprot.2 | Q62745 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Basolateral cell membrane; Multi-pass membrane protein . Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane. . |
| Tissue Expression | Expressed on B cells (at protein level) (PubMed:20237408). Expressed in hepatocytes (at protein level) (PubMed:12483205). Expressed in monocytes/macrophages (at protein level) (PubMed:12796480). Expressed on both naive and memory CD4-positive T cells (at protein level) (PubMed:22307619). |
| Signaling_pathway | Organismal Systems >> Immune system >> B cell receptor signaling pathway |
| Research Areas | >>B cell receptor signaling pathway; / >>Malaria; / >>Hepatitis C |
| Function | Function:May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.,PTM:Not glycosylated.,similarity:Belongs to the tetraspanin (TM4SF) family.,subunit:Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8.,tissue specificity:Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines., |
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