Price range: $268.00 through $328.00
Background:
This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013],
| Size | 50μL/100μL |
| Target | CDK5 Activator-binding C42 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 68kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDK5RAP1. AA range:251-300 |
| Specificity | CDK5 Activator-binding C42 Polyclonal Antibody detects endogenous levels of CDK5 Activator-binding C42 protein. |
| Gene Name | CDK5RAP1 |
| Protein Name | CDK5 regulatory subunit-associated protein 1 |
| Other Name | CDK5RAP1; / C20orf34; / CGI-05; / HSPC167; / CDK5 regulatory subunit-associated protein 1; / CDK5 activator-binding protein C42 |
| Species | Human |
| Gene ID | 51654 |
| Uniprot | Q96SZ6, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8BTW8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion . |
| Tissue Expression | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas (PubMed:10721722). Expressed in neurons of central nervous tissue (PubMed:10721722, PubMed:11882646).; [Isoform 5]: Mainly expressed in brain, placenta and testis.; [Isoform 6]: High expression in placenta and lung. |
| Signaling_pathway | |
| Research Areas | |
| Function | cofactor:Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.,Function:Specifically inhibits CDK5 activation by CDK5R1.,sequence Caution:Translated as Glu.,similarity:Belongs to the methylthiotransferase family. MiaB subfamily.,similarity:Contains 1 MTTase N-terminal domain.,similarity:Contains 1 TRAM domain.,subunit:Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Forms a complex with CDK5R1 and CDK5.,tissue specificity:Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas., |
