Price range: $268.00 through $328.00
Background:
This gene encodes a centrosomal protein that interacts with BRCA2, and is required for centriole duplication and cytokinesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011],
| Size | 50μL/100μL |
| Target | Centrobin |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 100kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CNTROB. AA range:591-640 |
| Specificity | Centrobin Polyclonal Antibody detects endogenous levels of Centrobin protein. |
| Gene Name | CNTROB |
| Protein Name | Centrobin |
| Other Name | CNTROB; / LIP8; / PP1221; / Centrobin; / Centrosomal BRCA2-interacting protein; / LYST-interacting protein 8 |
| Species | Human |
| Gene ID | 116840 |
| Uniprot | Q8N137, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8CB62 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Centriole-associated, asymmetrically localizes to the daughter centriole. |
| Tissue Expression | Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases.,Function:Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence Caution:Wrong choice of frame.,subcellular location:Centriole-associated, asymmetrically localizes to the daughter centriole.,subunit:Interacts with LYST.,tissue specificity:Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes., |
