U-Blot® Cofilin Rabbit mAb
WR4465 Western Blot analysis using C6 lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004],
Specifications
| Size | 50μL/100μL |
| Target | Cofilin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 19kD |
| MW(Observed) | 19kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:20000-1:50000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0685R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CFL1 CFL |
| Protein Name | Cofilin |
| Other Name | Cofilin-1; / 18 kDa phosphoprotein; / p18; / Cofilin, non-muscle isoform |
| Species | Human |
| Gene ID | 1072 |
| Uniprot | P23528, |
| Species.1 | Mouse |
| Gene ID.1 | 12631 |
| Uniprot.1 | P18760, |
| Species.2 | Rat |
| Gene ID.2 | 29271 |
| Uniprot.2 | P45592 |
| Cellular Localization | Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cell projection, growth cone . Cell projection, axon . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. |
| Tissue Expression | Widely distributed in various tissues. |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis |
| Research Areas | >>Axon guidance; / >>Fc gamma R-mediated phagocytosis; / >>Regulation of actin cytoskeleton; / >>Pertussis; / >>Human immunodeficiency virus 1 infection |
| Function | Function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues., |
Please login to post questions
Related products
