Price range: $268.00 through $328.00
Background:
CK4 is a cytokeratin with a molecular weight of 59 kDa. It is a member of type II cytokeratin family. CK4 is widely expressed in non keratinocytes and stratified squamous epithelium. It is mainly used to label esophageal and oral squamous epithelial cells and tumors of their origin.
| Size | 50μL/100μL |
| Target | Cytokeratin 4 |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 57kD |
| MW(Observed) | 54kD |
| Host Species | Mouse |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-CK4 |
| Immunogen | Synthesized peptide derived from human Cytokeratin 4 AA range: 400-534 |
| Specificity | The antibody can specifically recognize human CK4 protein, and shows no cross reaction with CK5, 6, 7, 8, 14, 15, 17, 20. |
| Gene Name | KRT4 CYK4 |
| Protein Name | Keratin, type II cytoskeletal 4 (Cytokeratin-4) (CK-4) (Keratin-4) (K4) (Type-II keratin Kb4) |
| Other Name | CK 4; / CK-4; / CK4; / CYK4; / Cytokeratin 4; / CytokeRatin-4; / CytokeRatin4; / FLJ31692; / K2C4_HUMAN; / K4; / KeRatin 4; / KeRatin; / KeRatin type II cytoskeletal 4; / KeRatin-4; / KeRatin4; / KRT 4; / Krt4; / type II cytoskeletal 4; / Type-II keRatin Kb4 |
| Species | Human |
| Gene ID | |
| Uniprot | P19013 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic, Membranous |
| Tissue Expression | Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,polymorphism:Three alleles of K4 are known: K4A2 (shown here), K4A1 and K4B.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13.,tissue specificity:Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer., |
