Cytokeratin 8 [ABT536] mouse mAb
Ucallm_WM1479-50
Price range: $268.00 through $328.00
Product details
Background:
Cytokeratin 8 (CK8) is a member of the type II family of cytokeratins with a molecular weight of 53 kDa which plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. It generally exists in monolayer and pseudostratified epithelium, and is expressed in glandular epithelium, transitional epithelium and hepatocytes. It is absent in squamous cell carcinomas. CK8 dimerizes with CK18 to form an intermediate filament in simple single-layered epithelial cells. CK8/18 expression patterns are useful for classification of tumors of unknown origin and poorly differentiated carcinomas.
Specifications
| Size | 50μL/100μL |
| Target | Cytokeratin 8 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 54kD |
| MW(Observed) | 54kD |
| Host Species | Mouse |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM536 |
| Immunogen | Synthesized peptide derived from human Cytokeratin 8 AA range: 400-483 |
| Specificity | The antibody can specifically recognize human CK8 protein, and shows no cross reaction with CK1, 4, 5, 6, 7, 10, 15, 16, 17, 18, 20. |
| Gene Name | KRT8 CYK8 |
| Protein Name | CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8 |
| Other Name | CARD2; / CK 8; / CK-8; / CK8; / CYK8; / CYKER; / Cytokeratin endo A; / CytokeRatin-8; / DreK8; / EndoA; / K2C8; / K2C8_HUMAN; / K8; / KeRatin 8; / KeRatin type II cytoskeletal 8; / KeRatin, type II cytoskeletal 8; / KeRatin-8; / KO; / Krt 2.8; / KRT8; / MGC118110; / MGC174782; / MGC53564; / MGC85764; / sb:cb186; / Type-II keRatin Kb8 |
| Species | Human |
| Gene ID | 3856 |
| Uniprot | P05787, |
| Species.1 | Mouse |
| Gene ID.1 | 16691 |
| Uniprot.1 | P11679, |
| Species.2 | Rat |
| Gene ID.2 | 25626 |
| Uniprot.2 | Q10758 |
| Cellular Localization | Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix . |
| Tissue Expression | Liver/ Tonsil |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].,Function:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.,tissue specificity:Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity., |
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