DAPK1 Polyclonal Antibody
Ucallm_WP11952-50
Price range: $268.00 through $328.00
Product details
Background:
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
Specifications
| Size | 50μL/100μL |
| Target | DAPK1 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 160kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DAPK1. AA range:274-323 |
| Specificity | DAPK1 Polyclonal Antibody detects endogenous levels of DAPK1 protein. |
| Gene Name | DAPK1 |
| Protein Name | Death-associated protein kinase 1 |
| Other Name | DAPK1; / DAPK; / Death-associated protein kinase 1; / DAP kinase 1 |
| Species | Human |
| Gene ID | 1612 |
| Uniprot | P53355, |
| Species.1 | Mouse |
| Gene ID.1 | 69635 |
| Uniprot.1 | Q80YE7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. |
| Tissue Expression | Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Bladder cancer |
| Research Areas | >>Autophagy - animal; / >>Pathways in cancer; / >>Bladder cancer |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,Function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system., |
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