Price range: $268.00 through $328.00
Background:
This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | DAPK2 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 43kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DAPK2. AA range:284-333 |
| Specificity | DAPK2 Polyclonal Antibody detects endogenous levels of DAPK2 protein. |
| Gene Name | DAPK2 |
| Protein Name | Death-associated protein kinase 2 |
| Other Name | DAPK2; / Death-associated protein kinase 2; / DAP kinase 2; / DAP-kinase-related protein 1; / DRP-1 |
| Species | Human |
| Gene ID | 23604 |
| Uniprot | Q9UIK4, |
| Species.1 | Mouse |
| Gene ID.1 | 13143 |
| Uniprot.1 | Q8VDF3 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle, autophagosome lumen. |
| Tissue Expression | Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Bladder cancer |
| Research Areas | >>Autophagy - animal; / >>Pathways in cancer; / >>Bladder cancer |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-318.,Function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.,tissue specificity:Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle., |
