Price range: $268.00 through $328.00
Background:
diacylglycerol kinase eta(DGKH) Homo sapiens This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014],
| Size | 50μL/100μL |
| Target | DGK-η |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 135kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKH. AA range:771-820 |
| Specificity | DGK-η Polyclonal Antibody detects endogenous levels of DGK-η protein. |
| Gene Name | DGKH |
| Protein Name | Diacylglycerol kinase eta |
| Other Name | DGKH; / Diacylglycerol kinase eta; / DAG kinase eta; / Diglyceride kinase eta; / DGK-eta |
| Species | Human |
| Gene ID | 160851 |
| Uniprot | Q86XP1 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Cell membrane . Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016). |
| Tissue Expression | [Isoform 1]: Expressed only in testis, kidney and colon.; [Isoform 2]: Ubiquitously expressed. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Glycerophospholipid metabolism; / >>Metabolic pathways; / >>Phosphatidylinositol signaling system; / >>Phospholipase D signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,Function:Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).,PTM:Phosphorylated; does not inhibit catalytic activity.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subcellular location:Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association.,subunit:Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity.,tissue specificity:Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon., |
