Price range: $268.00 through $328.00
Background:
docking protein 1(DOK1) Homo sapiens The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016],
| Size | 50μL/100μL |
| Target | p62 Dok |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 52kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p62 Dok. AA range:329-378 |
| Specificity | Dok-1 Polyclonal Antibody detects endogenous levels of Dok-1 protein. |
| Gene Name | DOK1 |
| Protein Name | Docking protein 1 |
| Other Name | DOK1; / Docking protein 1; / Downstream of tyrosine kinase 1; / p62; / dok; / pp62 |
| Species | Human |
| Gene ID | 1796 |
| Uniprot | Q99704, |
| Species.1 | Mouse |
| Gene ID.1 | 13448 |
| Uniprot.1 | P97465, |
| Species.2 | Rat |
| Gene ID.2 | 312477 |
| Uniprot.2 | Q4QQV2 |
| Cellular Localization | [Isoform 1]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm, perinuclear region. |
| Tissue Expression | Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer. |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:The PTB domain mediates receptor interaction.,Function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.,PTM:Constitutively tyrosine-phosphorylated.,PTM:Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.,similarity:Belongs to the DOK family. Type A subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with ABL (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3.,tissue specificity:Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells., |
