EHMT2 Polyclonal Antibody
Ucallm_WP2684-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],
Specifications
| Size | 50μL/100μL |
| Target | EHMT2 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 133kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 370-450 |
| Specificity | EHMT2 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | EHMT2 BAT8 C6orf30 G9A KMT1C NG36 |
| Protein Name | Histone-lysine N-methyltransferase EHMT2 (Euchromatic histone-lysine N-methyltransferase 2) (HLA-B-associated transcript 8) (Histone H3-K9 methyltransferase 3) (H3-K9-HMTase 3) (Lysine N-methyltransferase 1C) (Protein G9a) |
| Other Name | |
| Species | Human |
| Gene ID | 10919 |
| Uniprot | Q96KQ7, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9Z148 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Chromosome . Associates with euchromatic regions. Does not associate with heterochromatin. |
| Tissue Expression | Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. |
| Signaling_pathway | Organismal Systems >> Aging >> Longevity regulating pathway |
| Research Areas | >>Lysine degradation; / >>Metabolic pathways; / >>Longevity regulating pathway |
| Function | Alternative products:Additional isoforms seem to exist,Catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,Caution:It is uncertain whether Met-1 or Met-21 is the initiator methionine.,Caution:PubMed:11707778 reported that while NG36 and G9a were originally thought to derive from two separate genes, all G9A transcripts also contain the in frame coding sequence of NG36.,Domain:The SET domain mediates interaction with WIZ.,Function:Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.,similarity:Contains 1 post-SET domain.,similarity:Contains 1 pre-SET domain.,similarity:Contains 1 SET domain.,similarity:Contains 7 ANK repeats.,subcellular location:Associates with euchromatic regions. Does not associate with heterochromatin.,subunit:Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1.,tissue specificity:Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow., |
Please login to post questions
Related products
