Name: eIF2α Polyclonal Antibody
SKU: WP12139
Price: 268 USD
Availability: InStock

Product details

Background:

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],

Specifications

Size	50μL/100μL
Target	eIF2α
Reactivity	Human, Mouse, Rat, Monkey, Fish
Applications	IF, WB, IHC, ELISA
MW(Calculated)
MW(Observed)	38kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; Not yet tested in other applications; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human eIF2 alpha. AA range:21-70
Specificity	eIF2α Polyclonal Antibody detects endogenous levels of eIF2α protein.
Gene Name	EIF2S1
Protein Name	Eukaryotic translation initiation factor 2 subunit 1
Other Name	EIF2S1; / EIF2A; / Eukaryotic translation initiation factor 2 subunit 1; / Eukaryotic translation initiation factor 2 subunit alpha; / eIF-2-alpha; / eIF-2A; / eIF-2alpha
Species	Human
Gene ID	1965
Uniprot	P05198,
Species.1	Mouse
Gene ID.1	13665
Uniprot.1	Q6ZWX6,
Species.2	Rat
Gene ID.2	54318
Uniprot.2	P68101
Cellular Localization	Cytoplasm, Stress granule . Colocalizes with NANOS3 in the stress granules. .
Tissue Expression	B cells,Brain,Fibroblast,Placenta,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
Research Areas	>>Autophagy - animal; / >>Protein processing in endoplasmic reticulum; / >>Apoptosis; / >>Non-alcoholic fatty liver disease; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Hepatitis C; / >>Measles; / >>Influenza A; / >>Herpes simplex virus 1 infection; / >>Lipid and atherosclerosis
Function	Function:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.,PTM:Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus A, eIF2S1 phosphorylation state is modulated.,similarity:Belongs to the eIF-2-alpha family.,similarity:Contains 1 S1 motif domain.,subunit:Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.,

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eIF2α Polyclonal Antibody

Product details

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eIF2α Polyclonal Antibody

Product details

Specifications

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