U-Blot® Fascin Rabbit mAb
WR4301 SH-SY5Y whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011],
Specifications
| Size | 50μL/100μL |
| Target | Fascin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 54kD |
| MW(Observed) | 54kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0620R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | FSCN1 FAN1 HSN SNL |
| Protein Name | Fascin (55 kDa actin-bundling protein) (Singed-like protein) (p55) |
| Other Name | |
| Species | Human |
| Gene ID | 815 |
| Uniprot | Q16658, |
| Species.1 | Mouse |
| Gene ID.1 | 14086 |
| Uniprot.1 | Q61553, |
| Species.2 | Rat |
| Gene ID.2 | 683788 |
| Uniprot.2 | P85845 |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>ErbB signaling pathway; / >>Calcium signaling pathway; / >>cAMP signaling pathway; / >>HIF-1 signaling pathway; / >>Oocyte meiosis; / >>Necroptosis; / >>Adrenergic signaling in cardiomyocytes; / >>Wnt signaling pathway; / >>Axon guidance; / >>Circadian entrainment; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Cholinergic synapse; / >>Dopaminergic synapse; / >>Olfactory transduction; / >>Inflammatory mediator regulation of TRP channels; / >>Insulin secretion; / >>GnRH signaling pathway; / >>Melanogenesis; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Aldosterone synthesis and secretion; / >>Cushing syndrome; / >>Gastric acid secretion; / >>Parkinson disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Tuberculosis; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Glioma; / >>Diabetic cardiomyopathy; / >>Lipid and atherosclerosis |
| Function | Disease:Marks and mediates breast cancer metastasis to the lungs. FSCN1 is not functionally validated but achieves the highest statistical significance (P less than 0.000001). Those subjects expressing the lung metastasis signature have a significantly poorer lung metastasis-free survival, but not bone metastasis-free survival, compared to subjects without the signature.,Function:Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.,PTM:Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.,similarity:Belongs to the fascin family.,subunit:Associates with beta-catenin.,tissue specificity:Ubiquitous., |
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