Name: Fibrinogen γ Monoclonal Antibody
SKU: WM0192
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015],

Specifications

Size	50μL/100μL
Target	Fibrinogen γ
Reactivity	Human
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)	52kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	15D10
Immunogen	Purified recombinant fragment of human Fibrinogen γ expressed in E. Coli.
Specificity	Fibrinogen γ Monoclonal Antibody detects endogenous levels of Fibrinogen γ protein.
Gene Name	FGG
Protein Name	Fibrinogen gamma chain
Other Name	FGG; / Fibrinogen gamma chain
Species	Human
Gene ID	2266
Uniprot	P02679,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q8VCM7
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted .
Tissue Expression	Detected in blood plasma (at protein level).
Signaling_pathway	Organismal Systems >> Immune system >> Complement and coagulation cascades;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation
Research Areas	>>Complement and coagulation cascades; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>Staphylococcus aureus infection; / >>Coronavirus disease - COVID-19
Function	Disease:Defects in FGG are a cause of congenital afibrinogenemia [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.,Disease:Defects in FGG are a cause of thrombophilia.,Domain:A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.,Function:Fibrinogen has a double Function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.,miscellaneous:The gamma-chain carries the main binding site for the platelet receptor.,online information:Fibrinogen entry,PTM:Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.,PTM:Sulfation of C-terminal tyrosines increases affinity for thrombin.,similarity:Contains 1 fibrinogen C-terminal domain.,subunit:Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.,