Price range: $268.00 through $328.00
Background:
This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009],
| Target | GCNT3 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 51kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCNT3. AA range:226-275 |
| Sequence | |
| Specificity | GCNT3 Polyclonal Antibody detects endogenous levels of GCNT3 protein. |
| Gene Name | GCNT3 |
| Protein Name | Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 |
| Other Name | GCNT3; / Beta-1; / 3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; / C2GnT-mucin type; / C2GnT-M; / hC2GnT-M; / Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; / C2/4GnT |
| Species | Human |
| Gene ID-1 | 9245 |
| Uniprot | O95395, |
| Species.1 | Mouse |
| Gene ID-2 | |
| Uniprot.1 | Q5JCT0 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009], |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein . |
| Tissue Expression | Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced. |
| Signaling_pathway | |
| Research Areas | >>Mucin type O-glycan biosynthesis; / >>Metabolic pathways |
| Function | Catalytic activity:UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.,Catalytic activity:UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.,Function:Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.,induction:By all-trans retinoic acid (ATRA), TNF-alpha and IL13. Strongly down-regulated in colorectal cancer.,online information:Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,PTM:N-glycosylated.,similarity:Belongs to the glycosyltransferase 14 family.,tissue specificity:Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced., |
| RRID |
