Name: U-Blot® Glut4 Rabbit mAb
SKU: WR4947
Price: 268 USD
Availability: InStock

Product details

Background:This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Glut4
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	55kD
MW(Observed)	55kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1170R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	SLC2A4
Protein Name	Solute carrier family 2 facilitated glucose transporter member 4
Other Name	SLC2A4; / GLUT4; / Solute carrier family 2, facilitated glucose transporter member 4; / Glucose transporter type 4, insulin-responsive; / GLUT-4
Species	Human
Gene ID	6517
Uniprot	P14672,
Species.1	Mouse
Gene ID.1	20528
Uniprot.1	P14142,
Species.2	Rat
Gene ID.2	25139
Uniprot.2	P19357
Cellular Localization	Cell membrane; Multi-pass membrane protein . Endomembrane system; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized (PubMed:8300557). The dileucine internalization motif is critical for intracellular sequestration (PubMed:8300557). Insulin stimulation induces translocation to the cell membrane (By similarity). .
Tissue Expression	Skeletal and cardiac muscles; brown and white fat.
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>FoxO signaling pathway; / >>AMPK signaling pathway; / >>Insulin signaling pathway; / >>Adipocytokine signaling pathway; / >>Type II diabetes mellitus; / >>Insulin resistance; / >>Diabetic cardiomyopathy
Function	Disease:Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.,Function:Insulin-regulated facilitative glucose transporter.,miscellaneous:Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation.,online information:GLUT4 entry,PTM:Sumoylated.,similarity:Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.,subcellular location:Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.,subunit:Binds to DAXX. Interacts via its N-terminus with SRFBP1.,tissue specificity:Skeletal and cardiac muscles; brown and white fat.,