U-Blot® GSK3α Rabbit mAb
WR4183 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4183 Western blot analysis of lysates from Hela cells, using VASP Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
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Product details
Background:glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011],
Specifications
| Size | 50μL/100μL |
| Target | GSK3α |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 51kD |
| MW(Observed) | 51kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0615R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GSK3A |
| Protein Name | Glycogen synthase kinase-3 alpha |
| Other Name | GSK3A; / Glycogen synthase kinase-3 alpha; / GSK-3 alpha; / Serine/threonine-protein kinase GSK3A |
| Species | Human |
| Gene ID | 7408 |
| Uniprot | P49840, |
| Species.1 | Mouse |
| Gene ID.1 | 606496 |
| Uniprot.1 | Q2NL51, |
| Species.2 | Rat |
| Gene ID.2 | 50686 |
| Uniprot.2 | P18265 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Highly expressed in platelets. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>Focal adhesion; / >>Tight junction; / >>Platelet activation; / >>Fc gamma R-mediated phagocytosis; / >>Leukocyte transendothelial migration |
| Function | Catalytic activity:ATP + [tau protein] = ADP + [tau protein] phosphate.,Function:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer., |
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