Name: Hck Monoclonal Antibody
SKU: WM0232
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010],

Specifications

Size	50μL/100μL
Target	Hck
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	60kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	13B10
Immunogen	Purified recombinant fragment of Hck expressed in E. Coli.
Specificity	Hck Monoclonal Antibody detects endogenous levels of Hck protein.
Gene Name	HCK
Protein Name	Tyrosine-protein kinase HCK
Other Name	HCK; / Tyrosine-protein kinase HCK; / Hematopoietic cell kinase; / Hemopoietic cell kinase; / p59-HCK/p60-HCK; / p59Hck; / p61Hck
Species	Human
Gene ID	3055
Uniprot	P08631,
Species.1	Mouse
Gene ID.1
Uniprot.1	P08103
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	[Isoform 1]: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.; [Isoform 2]: Cell membrane; Lipid-anchor . Membrane, caveola; Lipid-anchor . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Golgi apparatus . Cytoplasmic vesicle . Lysosome . Nucleus . 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.; Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.
Tissue Expression	Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
Signaling_pathway	Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway
Research Areas	>>Chemokine signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Kaposi sarcoma-associated herpesvirus infection
Function	Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:The SH3 domain mediates binding to HIV-1 Nef.,Function:May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.,PTM:Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:May interact (via SH3 domain) with HIV-1 Nef and Vif. This interaction would stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein.,tissue specificity:Expressed predominantly in cells of the myeloid and B-lymphoid lineages.,

Please login to post questions

Hck Monoclonal Antibody

Product details

Specifications

About Us

Support

Order

Menu

Hck Monoclonal Antibody

Product details

Specifications

Related products

U-Blot® Smad2 (Phospho Ser250) Rabbit mAb

U-Blot® MMP7 Rabbit mAb

U-Blot® GAPDH Rabbit mAb

U-Blot® GSK3 β (Phospho Ser9) Rabbit mAb

U-Blot® BID Rabbit mAb

U-Blot® Hsp90 Rabbit mAb

Customer Reviews

Menu