U-Blot® Histone H2A.X (Phospho Ser139) Rabbit mAb

Price range: $288.00 through $368.00

SKU: WR4174-50
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Product details

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],

Specifications

TargetHistone H2A.X
ReactivityHuman,Mouse,Rat,
ApplicationWB,IHC,IF,IP,ELISA
MW(Calculated)15kD
MW(Observed)15kD
Host SpeciesRabbit
IsotypeIgG,Kappa
ConjugateUnconjugated
ModificationPhospho
Modification siteSer139
Recommended dilution ratioIHC 1:2000-1:8000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200,
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
PurificationProtein A
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityRecombinant Monoclonal
Clone NumberPT0330R
Immunogen
Sequence
SpecificityThis antibody detects endogenous levels of Histone H2A.X only when phosphorylated at Ser139.This antibody does not recognize phosphorylated at other sites.
Gene nameH2AFX
Protein nameHistone H2A.x
Alternative nameH2AFX;H2AX;Histone H2A.x;H2a/x
Organism-1Human
Gene ID‑13014
SwissProt-1P16104
Organism-2Mouse
Gene ID‑215270
SwissProt-2P27661
Organism-3
Gene ID‑3
SwissProt-3
Organism-4
Gene ID‑4
SwissProt-4
Cellular LocalizationNucleus
Unnamed: 38
Unnamed: 39
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