U-Blot® Histone H2A.X Rabbit pAb
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Price range: $268.00 through $328.00
Product details
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],
Specifications
| Target | Histone H2A.X |
| Reactivity | Human,Mouse,Rat |
| Application | WB,IHC,IF,CoIP,ELISA |
| MW(Calculated) | |
| MW(Observed) | 19kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Modification | Unmodified |
| Modification site | |
| Recommended dilution ratio | WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;IP 1:200-500;ELISA 1:10000;Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H2A.X. AA range:94-143 |
| Sequence | |
| Specificity | Histone H2A.X Polyclonal Antibody detects endogenous levels of Histone H2A.X protein. |
| Gene name | H2AFX |
| Protein name | Histone H2A.x |
| Alternative name | H2AFX;H2AX;Histone H2A.x;H2a/x |
| Organism-1 | Human |
| Gene ID‑1 | 3014 |
| SwissProt-1 | P16104 |
| Organism-2 | Mouse |
| Gene ID‑2 | 15270 |
| SwissProt-2 | P27661 |
| Organism-3 | |
| Gene ID‑3 | |
| SwissProt-3 | |
| Organism-4 | |
| Gene ID‑4 | |
| SwissProt-4 | |
| Cellular Localization | Nucleus . Chromosome . |
| Unnamed: 38 | |
| Unnamed: 39 |
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