Name: HNF-4α/γ Polyclonal Antibody
SKU: WP12709
Price: 268 USD
Availability: InStock

Product details

Background:

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012],

Specifications

Size	50μL/100μL
Target	HNF-4α/γ
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	52kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human HNF4 alpha/gamma. AA range:91-140
Specificity	HNF-4α/γ Polyclonal Antibody detects endogenous levels of HNF-4α/γ protein.
Gene Name	HNF4A/HNF4G
Protein Name	Hepatocyte nuclear factor 4-alpha/gamma
Other Name	HNF4A; / HNF4; / NR2A1; / TCF14; / Hepatocyte nuclear factor 4-alpha; / HNF-4-alpha; / Nuclear receptor subfamily 2 group A member 1; / Transcription factor 14; / TCF-14; / Transcription factor HNF-4; / HNF4G; / NR2A2; / Hepatocyte nuclear factor 4-gamma; / HNF-4-ga
Species	Human
Gene ID	3172;3174
Uniprot	P41235;Q14541,
Species.1	Mouse
Gene ID.1	15378;30942,
Uniprot.1
Species.2	Rat
Gene ID.2	25735
Uniprot.2	P22449
Cellular Localization	Nucleus.
Tissue Expression	Kidney,Liver,
Signaling_pathway	Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>AMPK signaling pathway; / >>Maturity onset diabetes of the young
Function	Alternative products:Additional isoforms seem to exist,Disease:Defects in HNF4A are the cause of maturity onset diabetes of the young type 1 (MODY1) [MIM:125850]; also shortened MODY-1. MODY [MIM:606391] is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age) and a primary defect in insulin secretion. The clinical phenotype of MODY1 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications.,Function:Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.,miscellaneous:Binds fatty acids.,online information:Hepatocyte nuclear factors entry,PTM:Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR2 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Homodimerization is required for HNF4-alpha to bind to its recognition site.,