Price range: $268.00 through $328.00
Background:
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | HSL |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 85kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSL. AA range:518-567 |
| Specificity | HSL Polyclonal Antibody detects endogenous levels of HSL protein. |
| Gene Name | LIPE |
| Protein Name | Hormone-sensitive lipase |
| Other Name | LIPE; / Hormone-sensitive lipase; / HSL |
| Species | Human |
| Gene ID | 3991 |
| Uniprot | Q05469, |
| Species.1 | Mouse |
| Gene ID.1 | 16890 |
| Uniprot.1 | P54310, |
| Species.2 | Rat |
| Gene ID.2 | 25330 |
| Uniprot.2 | P15304 |
| Cellular Localization | Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). . |
| Tissue Expression | Testis. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>cAMP signaling pathway; / >>AMPK signaling pathway; / >>Apelin signaling pathway; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Aldosterone synthesis and secretion |
| Function | Catalytic activity:Diacylglycerol + H(2)O = monoacylglycerol + a carboxylate.,Catalytic activity:Monoacylglycerol + H(2)O = glycerol + a carboxylate.,Catalytic activity:Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.,enzyme regulation:Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.,Function:In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.,pathway:Glycerolipid metabolism; triacylglycerol degradation.,similarity:Belongs to the 'GDXG' lipolytic enzyme family.,subcellular location:Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.,subunit:Interacts with PTRF in the adipocyte cytoplasm., |
