Price range: $268.00 through $328.00
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009],
| Size | 50μL/100μL |
| Target | HXK II |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 102kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 5F10 |
| Immunogen | Purified recombinant fragment of human HXK II expressed in E. Coli. |
| Specificity | HXK II Monoclonal Antibody detects endogenous levels of HXK II protein. |
| Gene Name | HK2 |
| Protein Name | Hexokinase-2 |
| Other Name | HK2; / Hexokinase-2; / Hexokinase type II; / HK II; / Muscle form hexokinase |
| Species | Human |
| Gene ID | 3099 |
| Uniprot | P52789, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O08528 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion outer membrane; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (PubMed:29298880). The interaction with the mitochondrial outer membrane via the mitochondrial-binding peptide (MBP) region promotes higher stability of the protein (PubMed:29298880). Release from the mitochondrial outer membrane into the cytosol induces permeability transition pore (PTP) opening and apoptosis (PubMed:18350175). . |
| Tissue Expression | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Fructose and mannose metabolism; / >>Galactose metabolism; / >>Starch and sucrose metabolism; / >>Amino sugar and nucleotide sugar metabolism; / >>Neomycin, kanamycin and gentamicin biosynthesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of nucleotide sugars; / >>HIF-1 signaling pathway; / >>Insulin signaling pathway; / >>Type II diabetes mellitus; / >>Carbohydrate digestion and absorption; / >>Shigellosis; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,Domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,polymorphism:Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle., |
