Price range: $268.00 through $328.00
Background:
This gene encodes an intracellular receptor for inositol 1, 4, 5-trisphosphate. Upon stimulation by inositol 1, 4, 5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009],
| Target | IP3 Receptor |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 320kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from IP3R-I . at AA range: 1700-1780 |
| Sequence | |
| Specificity | IP3R-I Polyclonal Antibody detects endogenous levels of IP3R-I protein. |
| Gene Name | ITPR1 INSP3R1 |
| Protein Name | Inositol 1,4,5-trisphosphate receptor type 1 |
| Other Name | ITPR1; / INSP3R1; / Inositol 1,4,5-trisphosphate receptor type 1; / IP3 receptor isoform 1; / IP3R 1; / InsP3R1; / Type 1 inositol 1,4,5-trisphosphate receptor; / Type 1 InsP3 receptor |
| Species | Human |
| Gene ID-1 | 3708 |
| Uniprot | Q14643, |
| Species.1 | Mouse |
| Gene ID-2 | 16438 |
| Uniprot.1 | P11881, |
| Species.2 | Rat |
| Gene ID-3 | 25262 |
| Uniprot.2 | P29994 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009], |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein . Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein . Cytoplasm, perinuclear region . Endoplasmic reticulum and secretory granules (By similarity). . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>Phosphatidylinositol signaling system; / >>Oocyte meiosis; / >>Autophagy - animal; / >>Apoptosis; / >>Cellular senescence; / >>Vascular smooth muscle contraction; / >>Apelin signaling pathway; / >>Gap junction; / >>Platelet activation; / >>NOD-like receptor signaling pathway; / >>C-type lectin receptor signaling pathway; / >>Circadian entrainment; / >>Long-term potentiation; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>Dopaminergic synapse; / >>Long-term depression; / >>Inflammatory mediator regulation of TRP channels; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Thyroid hormone synthesis; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Renin secretion; / >>Aldosterone synthesis and secretion; / >>Cortisol synthesis and secretion; / >>Parathyroid hormone synthesis, secretion and action; / >>GnRH secretion; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Salivary secretion; / >>Gastric acid secretion; / >>Pancreatic secretion; / >>Alzheimer disease; / >>Parkinson disease; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Shigellosis; / >>Human cytomegalovirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Proteoglycans in cancer; / >>Lipid and atherosclerosis |
| Function | Alternative products:There is a combination of three alternatively spliced domains at site SI, SIII and site SII (A and C). Experimental confirmation may be lacking for some isoforms,Disease:Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.,Domain:The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.,Function:Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.,miscellaneous:Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor.,PTM:Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.,PTM:Phosphorylated on tyrosine residues.,similarity:Belongs to the InsP3 receptor family.,similarity:Contains 5 MIR domains.,subunit:Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with AHCYL1 (By similarity). Interacts with MRVI1.,tissue specificity:Widely expressed., |
| RRID |
